Effects of microwave radiation on learning and memory and neurotransmitters in the hippocampus / 中国辐射卫生

@#The central nervous system is one of the most sensitive targets of microwave radiation. Microwave radiation can affect spatial learning and memory and neural information transmission. The effects of microwave radiation on neurotransmitters in the hippocampus and the underlying mechanisms are still unclear. This paper reviews the effects of microwave radiation on learning/memory and neurotransmitters as well as the mechanisms of action on neurotransmitters. This paper aims to provide a scientific basis for future research in this area.

Preliminary exploration on operation process for autologous ozonized blood transfusion / 中国输血杂志

Autologous ozonized blood transfusion(AOBT) is a therapy of re-transfusion of 100-200 mL of autologous blood after shaking and agitation with appropriate amount of oxygen-ozone in vitro. The oxidation of blood through the strong oxidation of ozone can enhance the non-specific immune response of the body, regulate the internal environment and promote health. This therapy has been increasingly applied in clinical practice, while no unified standard for the operation process in terms of ozone concentration, treatment frequency and treatment course had been established. This operation process of AOBT is primarily explored in order to standardize the operation process and ensure its safety and efficacy.

Effects of 5.8 GHz radiofrequency radiation on learning and memory along with synaptic plasticity of hippocampal neurons in rats / 中华放射医学与防护杂志

Objective:To investigate the effect of 5.8 GHz radiofrequency (RF) radiation on learning and memory along with hippocampal synaptic plasticity in rats, in order to provide theoretical and experimental references for scientific evaluation of potential hazards of 5.8 GHz RF radiation.Methods:A total of 56 healthy adult male Sprague-Dawley rats were randomly divided into sham exposure group ( n=28) and RF exposure group ( n=28). RF groups were exposed to 5.8 GHz RF for 1 h each day in 15 d or 30 d continuously, and the whole-body absorption rate was 1.15 W/kg. The learning and memory ability of rats was tested by Morris water maze (MWM). The hippocampal structure of rats was observed by Nissl stain. The density of dendritic spines in CA1 region of hippocampus was detected by Golgi stain. The expression of synaptic related protein (PSD95, Synaptophysin) in hippocampus was detected by Western blot. The level of hippocampal neurotransmitters was detected by liquid chromatography-mass spectrometry. Results:In MWM experiments, at 15 d and 30 d after RF exposure, there was no statistically significant difference between sham group and RF group in the escape latency, frequency of crossing plateau, percentage of stay time in plateau quadrant and latency of first arrival to the plateau ( P>0.05). Besides, the structure and the number of neurons in the hippocampus, the density of apical and basal dendritic spines of pyramidal neurons in the CA1 region (apical: 5.10±0.20, 4.89±0.24, 4.58±0.27, 4.49±0.24, and basal: 4.81±0.17, 4.79±0.34, 4.20±0.27, 4.22±0.17, named as Sham 15 d group, RF 15 d group, Sham 30 d group, RF 30 d group, respectively), the expression of PSD95 and Synaptophysin and the level of multiple kinds of neurotransmitters in the hippocampus had no significant changes ( P>0.05). Conclusions:In this study, 5.8 GHz RF radiation has no significant influence on the spatial learning and memory ability along with the synaptic plasticity of hippocampal neurons of rats.

Effects of electromagnetic pulse on reproduction of adult male mice / 中华放射医学与防护杂志

Objective To investigate the effects of electromagnetic pulse (EMP) on reproductive function of male adult mice.Methods A total of 48 healthy adult male BALB/c mice (8 weeks old) were randomly divided into sham group and EMP group with 24 animals in each group.The mice were wholebody exposed or sham exposed to EMP at 720 kV/m for 100 pulses with 1 Hz repetition rate and 40 ns pulse width.At 1,7,14 and 35 d after EMP exposure,the mice were anesthetized and the sperms were collected from the bilateral epididymal tail.After that,the sperm quality including the number of sperms,the ratio of abnormalities and the survival rate was evaluated.In addition,the morphology of testis was observed by HE staining and the diameter of seminiferous tubules was measured by Image J 1.43 u software.The protein level of stem cell factor (SCF) and glial-derived neurotrophic factor (GDNF) in testis tissue were detected by ELISA and Western blot.Results The sperm quality and the morphology of testis did not change obviously at different times after exposing mice to EMP at 720 kV/m for 100 pulses,compared with sham group (P＞0.05).The diameters of seminiferous tubules at 1,7,14 and 35 d after exposure were (196.85+ 16.65),(196.79+ 14.33),(196.35±22.71) and (198.60±25.88) μm in exposed mice,respectively,while (204.31±27.13),(197.07± 18.11),(194.37±21.45) and (200.59± 19.36) Iμm in sham exposed mice,respectively.There was no significant difference between two groups (P＞0.05).Additionally,the levels of SCF and GDNF in testis tissue between EMP group and sham group had no statistically significant difference (P＞0.05).Conclusion Under this exposure condition,EMP couldn't affect the reproductive function of male adult mice.

Detection of Transfusion Transmitted Diseases among Recipients Before Transfusion and Volunteer Donors:A Comparative Analysis of Results / 中华医院感染学杂志

OBJECTIVE To learn the relationship between blood recipients with transfusion transmitted diseases before transfusion volunteer donors.METHODS HBsAg,HCV antibody,HIV antibody and syphilis antibody in recipients and donorst were tested.RESULTS From 11 037,HBsAg was positive in 1019 cases(9.233%),anti-HCV positive in 61 cases(0.553%),anti-HIV was positive in 2 cases(0.018%),and syphilis was positive in 88 cases(0.797%).From 57 794 donors,HBsAg was positive(0.606%) in 350 cases,anti-HCV positive in 163 cases(0.282%),anti-HIV positive in 4 cases(0.007%),and syphilis was positive in 347 cases(0.600%).CONCLUSIONS The positive rates of HBsAg,HCV antibody and syphilis antibody among recipients are higher than that in blood donors.So,it′s reasonably to detect these serum infection markers in recipients before transfusion for the purpose of reducing or eliminating the occurrence of iatrogenal transfusion transmitted infection.

Blood Infection Marker Detection in Pregnant Women Before Delivery and Their Clinical Significance / 中华医院感染学杂志

OBJECTIVE To investigate the significance of the blood infection marker detection in pregnant women before delivery.METHODS From Jan to Oct 2007,a total of 2010 cases pregnant women were tested the HBsAg,HCV antibody,HIV1/2 antibody and RPR.RESULTS From them HBsAg was positive in 185 cases,the positive rate was 9.2%;4 cases was with positive HCV antibody,the positive rate was 0.2%;HIV1/2 antibody was not tested out;10 cases was with positive RPR antibody,the positive rate was 0.5%.The total positive rate was 9.9%.CONCLUSIONS The prenatal infection rates of hepatitis B,hepatitis C and RPR in the pregnant women show high rates which show the possible vertical transmission.It is essential to test infection marker in the pregnant women and,take effective measures.